No British women will feature in the main draw at the French Open – the first time this has happened in a Grand Slam since the US Open 2009.
Katie Boulter, Harriet Dart and Fran Jones were eliminated in the second qualifying round, after Heather Watson, Katie Swan and Lily Miyazaki who missed out on a place in the main tournament next week.
Emma Raducanu is absent from the event after undergoing ankle and wrist surgery.
Boulter lost a match in three sets to American Ashlyn Krueger on Wednesday, beaten 6-2 2-6 7-6 (10-3), while Dart fell to Frenchwoman Elsa Jacquemot 1-6 6-1 6 -2 because she was unable to build on a full first set.
Jones had beaten two-time Grand Slam semi-finalist Coco Vandeweghe on Monday, but saw her hopes of reaching the main stage at Roland Garros ended when she retired injured with a shoulder injury against Switzerland’s Ylena In-Albon.
Emma Raducanu is out of action after undergoing wrist and ankle surgery
Cameron Norrie, Dan Evans, Kyle Edmund and Jack Draper will be the only British players to feature in singles at the second Grand Slam of the year.
Andy Murray withdrew to prioritize the grass season, while Liam Broady, Jan Choinski and Ryan Peniston lost in qualifying.
The French Open draw will take place on Thursday afternoon with Carlos Alcaraz seeded in the men’s tournament and Iga Swiatek taking the women’s spot.